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Technologist, DTPPD

Toronto, Canada



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Position Title: Technologist

Type of Employment: Fixed-Term Contract – 12 Months

Site/Department: Toronto, Ontario / DTPPD Upstream

Major Job Activities:

Seedwork (aseptic technique, dispensing, purity testing, gram staining):

  • Primary operator for creation of Working Cell Bank, Glycerol Production Seed
  • Primary operator for lyophile/cryovial seeding, seed propagation aerobic and anaerobic cultures into solid/liquid media
  • Primary operator for sterile dispensing medium, media components
  • Execution of purity testing, gram staining, examination of cellular morphology phenotype
  • Seed enumeration analysis

Fermentation (SIP, DIP, pressurized vessel operation):

  • Primary operator confirm fermenter integral, ready for operation
  • Primary operator performing fermenter pressure testing at various ports (vessel, harvest, sample port)
  • Control of SIP for fermenter (automation and manual), confirming all temperature probes meet specific temperatures to achieve fermenter sterilization
  • Primary operator to confirm fermenter seed inoculation, at specified temperature, and monitoring duration
  • Primary operator ensuring correct media supplements added in specific order while monitoring vessel pressure

Filtration, Detoxification:

  • Primary operator for the inspection and release of purification equipment for toxin processing
  • Primary operator for the assembly of clarification train, ensuring correct assembly for processing of 1100L tetanus toxin
  • Primary operator to ensure depth filters assembled correctly, with all connections verified from fermenter to collection vessel
  • Primary operator for the critical addition of protein precipitation reagents
  • Primary operator for the recovery of precipitated toxin via centrifugation, and subsequent toxin re-suspension

Protein/cell concentration assay (Lf, OD) and Absence Test:

  • Primary operator for determination of final harvest yield
  • Determination of yield post filtration, and/or dialysis
  • Specific Toxicity Testing Lf set-up
  • Measurement culture OD and supernatant OD
  • Absence Testing post-0.2um filtration or post-cell inactivation procedures through bottom harvest valve

Documentation of activities and SPC Data Entry:

  • Concurrent BPR entry and checking of calculations
  • Data entry into SPC database for SPC trending
  • Organization of batch records
  • Monitoring of cleanroom, incubator, and refrigerator logs
  • Information retrieval for inspection, validation

SAP and EM Data Entry:

  • Data entry for SAP manufacturing processes and maintenance orders
  • Issuance and creation of process orders
  • Maintenance of material adjustment forms
  • Maintenance of inventory
  • Re-order of non-MRP items required for production activities

No employee has a proprietary right to an activity or group of activities. Activities may be changed or reassigned to meet the needs of the operations.

Minimum Qualifications:

  • Minimum four (4) years Bachelor’s Degree (Biology, Chemistry, and related sciences), plus a minimum one (1) year of previous relevant work experience.
  • Working knowledge in the following areas: Aseptic and Clean room Techniques; Fermentation, Pressurized vessels & processing techniques, Protein Purification
  • Excellent communication and teamwork skills
  • Strong Computer knowledge and familiarity with: RAPID, FMS, BAS, MASTER, PHARM-NET-WEB and Microsoft Office
  • Highly organized with the ability to effectively troubleshoot issues as they may arise

Hours Of Work:

Inclusive from Monday to Friday 7:00a.m. to 3:00p.m.

This is subject to change at any time and it should not be construed as limiting the right of the company to make such change to meet the requirements of the operation.

Sanofi Pasteur: The world’s leading vaccine company

Sanofi Pasteur, the vaccines division of Sanofi, is the largest company in the world devoted entirely to human vaccines. Our driving goal is to protect people from infectious diseases by creating safe and effective vaccines.Our company distributes more than 1 billion doses of vaccine each year, making it possible to vaccinate more than 500 million people across the globe.Sanofi Pasteur offers the broadest range of vaccines in the world, providing protection against 20 bacterial and viral diseases.

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Sanofi is an equal opportunity employer committed to diversity and inclusion. Our goal is to attract, develop and retain highly talented employees from diverse backgrounds, allowing us to benefit from a wide variety of experiences and perspectives. We welcome and encourage applications from all qualified applicants.  Accommodations for persons with disabilities required during the recruitment process are available upon request.

Thank you in advance for your interest.

Only those candidates selected for interviews will be contacted.

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At Sanofi diversity and inclusion is foundational to how we operate and embedded in our Core Values. We recognize to truly tap into the richness diversity brings we must lead with inclusion and have a workplace where those differences can thrive and be leveraged to empower the lives of our colleagues, patients and customers. We respect and celebrate the diversity of our people, their backgrounds and experiences and provide equal opportunity for all.

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