Post-doctoral Associate, Cell Science and Technology
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About the job
Job title: Post-doctoral Associate, Cell Science and Technology
Hiring Manager: Tiffany McLamarrah
Location: Framingham, MA, On-site, less than 5% travel required
Job Type: Permanent, Temporary, Full time,
The successful applicant will join the Cell Science and Technology team, who’s mission is to optimize Sanofi’s stable cell line Chinese Hamster Ovary (CHO) expression platform. To accomplish this, our team routinely employs genetic engineering of cell lines, as well as advanced molecular biology techniques in support of cell line generation and characterization, with the purpose of improving production of next generation biotherapeutics.
Generation of CHO cell lines stably expressing candidate biotherapeutics via random integration, transposase mediated integration, or recombinase mediated integration of transgenes
Perform genetic and phenotypic characterization of CHO cell lines, including transgene copy number and transcript analysis, cell growth, and protein production
Execute various molecular techniques for assembly of expression vectors, including Gibson assembly, PCR, restriction endonuclease digestions, DNA plasmid preparations, etc.
Use of basic sequence analysis tools (BLAST, Geneious, etc.) to analyze vector and genomic nucleotide sequences
Prepare reports, presentations, and publications to effectively communicate plans, progress, and results.
PhD +0-3 years of postdoctoral training
All candidates must have permanent US work authorization. No visa sponsorship will be provided
Experience with mammalian cell culture techniques, cell line engineering, and molecular biology
Technical skills: aseptic technique, cell counting, passaging, cryopreservation, transfection, Gibson assembly, PCR, DNA/RNA isolation, ddPCR
Experience with bioinformatics and data analysis – analysis of RNAseq and next gen sequencing data (short and long read data)
Previous first-author publication(s) in internationally recognized journals
Ability to meet aggressive timelines and deliverables in a collaborative environment
Excellent speaking, reading and writing skills
Strong analytical and organizational skills
Curiosity and willingness to work independently
Pursue progress, discover extraordinary
Better is out there. Better medications, better outcomes, better science. But progress doesn’t happen without people – people from different backgrounds, in different locations, doing different roles, all united by one thing: a desire to make miracles happen. So, let’s be those people.
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